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KMID : 1123920020160051016
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 5 p.1016 ~ p.1019
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Effect of Ramulus et uncus uncariae on Glucose Oxidase-Induced Toxicity in Cultured Cerebral Neurons
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Kim Hyung-Soo
Lee Yong-Suk
Oh Suk-Kyu
Lee Kang-Chang
Lee Geon-Mok
Lee Jeong-Won
Lee Sang-Bork
Kim Jong-Ho
Yu Jun-Ki
Kang Young-Seong
Kim Sung-Soo
Song Ho-Jun
Park Seung-Taeck
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Abstract
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To examine the cytotoxic effect of glucose oxidase(GO) in cultured mouse cerebral neurons, cytotoxicity was measured by MTT assay after cultured nerve cells were incubated for 3 hours in the media containing 1 ～ 60mU/ml concentrations of GO. In addition, the neuroprotective effect of Ramulus et uncus uncariae(REUU) was determined by MTT assay in these cultrures. Cell viability was remarkably decreased in a dose- and time-dependent manner after cultured mouse cerebral neurons were exposed to 30mU/ml GO for 3 hours. In the neuroprotective effect of REUU on GO-induced toxicity, REUU blocked the GO-mediated neurotoxicity in these cultures. From above the results, it suggests that GO is toxic in cultured mouse cerebral neurons and selective herb extract such as REUU is effective in prevetion of the neurotoxicity induced by GO.
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KEYWORD
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Cultured cerebral neuron, Ramulus et uncus uncariae, Neuroprotective effect
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